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The Lymphocyte Proliferation Assay (LPA) is a test used to measure the ability of lymphocytes to proliferate in response to various stimuli such as candida, pokeweed and phytohaemagglutinin. These plant lectins are carbohydrate binding proteins that bind to cell surface receptors and activate cells in an antigen independent manner. Most people will respond to at least one of several common microbial antigens. LPA can be used to measure improvements in immunological function following antiretroviral therapy and to detect the presence of immune responses against specific opportunistic pathogens.
The 3H-thymidine incorporation assay is used to determine the extent of cell division in response to a proliferation signal. 3H-thymidine which is a radioactive nucleoside and precursor of thymine found in DNA becomes incorporated in DNA strands of proliferating cells and can be measured to determine extent of cell proliferation.
Lab 15. Lymphocyte Proliferation Assay
Lymphocyte proliferation assay is used to determine lymphocyte activation and the cell-mediated immune responses. When B cells encounter their specific antigens, with the help of T cells, B cells are stimulated to undergo proliferation. When T cells are activated by antigen-presenting cells and cytokines, T cells undergo proliferation. The proliferation of B and T cells leads to clonal expansion and the initiation of the specific immune responses.
Cells undergoing proliferation increase their rate of
The 3H-thymidine incorporation assay is used to determine the extent of cell division in response to a proliferation signal. 3H-thymidine which is a radioactive nucleoside and precursor of thymine found in DNA becomes incorporated in DNA strands of proliferating cells and can be measured to determine extent of cell proliferation.
Lab 15. Lymphocyte Proliferation Assay
Lymphocyte proliferation assay is used to determine lymphocyte activation and the cell-mediated immune responses. When B cells encounter their specific antigens, with the help of T cells, B cells are stimulated to undergo proliferation. When T cells are activated by antigen-presenting cells and cytokines, T cells undergo proliferation. The proliferation of B and T cells leads to clonal expansion and the initiation of the specific immune responses.
Cells undergoing proliferation increase their rate of
